Device

Part:BBa_K3024011:Design

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-10-05)


Inducible cox characterization construct (Lytic module)

This part drives arabinose-dependent expression of Cox protein that is fused with cMyc tag for immunological detection, the TetR repressor and GFP as a live-cell expression reporter. This module was designed to experimentally induce the switch from lysogeny to lytic cycle and it is designed to be co-transfected with the part BBa_K3024009 which expresses C protein and is repressible by tetR. For more information visit https://2019.igem.org/Team:Stockholm.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1119
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 204
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1078
    Illegal BsaI.rc site found at 1785


Design Notes

Designed to meet complexity standards for IDT synthesis.

Source

Assembled from different parts derived from the igem registry, NCBI and uniprot. For details, see the respective basic parts.

References

Christie GE, Calendar R. Bacteriophage P2. Bacteriophage. 2016;6(1):e1145782.